Journal: Drug Delivery

Article Title: Heparan sulfate targeting strategy for enhancing liposomal drug accumulation and facilitating deep distribution in tumors

doi: 10.1080/10717544.2020.1745326

Figure Lengend Snippet: HS-dependent cellular uptake of GBP-modified pegylated liposomes. (A) The A549 cells were incubated with DiI-labeled liposomes (L-DIL) and GBP-modified L-DIL (L-DIL-GBP) at 37 °C for 4 h and were monitored by confocal microscopy. (B) The cells were incubated with L-DOX and L-DOX-GBP at 37 °C for 24 h, and cellular uptake and drug release were assessed by flow cytometry. (C) The cells were treated for 24 h with L-DOX-GBP (H) in the presence of the indicated concentrations of GAGs, including high-molecular-weight heparin (HMWH), low-molecular-weight heparin (LMWH), chondroitin sulfate type B (CS), and hyaluronic acid (HA), and the cellular uptake of doxorubicin was analyzed. The cellular uptake of the L-DOX treatment group was used to normalize that of the L-DOX-GBP(H) group upon GAG competition. (D) The cells were separately treated with 10 μM L-DOX and L-DOX-GBP at 37 °C for 24 h, and cellular uptake and drug release were assessed by fluorescence microscopy. Magnification: 40×; scale bar: 50 μm. The data are the mean ± SD, averaged from three separate experiments. * p < .05; ** p < .01, two-tailed Student’s t -test.

Article Snippet: Each image was measured by CLSM Z-stack scanning (pinhole: 1.7 μm; Z interval: 1.0 μm between consecutive slides) using a JelloX Biotech system.

Techniques: Modification, Liposomes, Incubation, Labeling, Confocal Microscopy, Flow Cytometry, High Molecular Weight, Molecular Weight, Fluorescence, Microscopy, Two Tailed Test

Journal: Drug Delivery

Article Title: Heparan sulfate targeting strategy for enhancing liposomal drug accumulation and facilitating deep distribution in tumors

doi: 10.1080/10717544.2020.1745326

Figure Lengend Snippet: Drug penetration activity of L-DIL-GBP in spheroids. Heterospheroids composed of A549 and NIH-3T3 cells were incubated with different formulations of L-DIL for 4 h. (A) Penetration capacity was measured by CLSM Z-stack scanning with pinhole: 1.7 μm; Z interval: 1.0 μm between consecutive slides. Nuclei stained by SYTO16; DiI (red). Magnification: 20×; scale bar: 100 μm. (B) Three-dimensional images were reconstructed to illustrate L-DIL or L-DIL-GBP(H) penetration into the heterospheroids. DiI signal (green); nucleus (red). (C) Quantitative analysis between the mean intensity of the Dil signal and the distance to the center of the spheroid. The data are the mean ± SD, averaged from three separate experiments. *, **, and *** indicate p < .05, p < .01 and p < .001 under the two-tailed t -test, respectively.

Article Snippet: Each image was measured by CLSM Z-stack scanning (pinhole: 1.7 μm; Z interval: 1.0 μm between consecutive slides) using a JelloX Biotech system.

Techniques: Activity Assay, Incubation, Staining, Two Tailed Test